PFKL ELISA Kit (Rat) (OKEH03584)

Catalog No: OKEH03584

Size:96T

Rat PFKL ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology PFKL ELISA Kit (Rat) (OKEH03584) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Pfkl antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Pfkl and incubated. The Pfkl found in the sample or standards competes with the biotinylated Pfkl for limited binding sites on the immobilized anti-human Pfkl antibody. Excess unbound biotinylated Pfkl and sample or standard Pfkl is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Pfkl captured in the well and inversely proportional to the amount of Pfkl which was contained in the sample or standard.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Component:
  • Anti-Pfkl Microplate: 96 Wells (12 x 8 Well strips)
  • Pfkl Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Pfkl-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.091 ng/mL
• Specificity: Natural and recombinant Rat 6-phosphofructokinase, liver type
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Pfkl
• Gene Full Name: phosphofructokinase, liver type
• Alias Symbols: 6-phosphofructokinase type B;6-phosphofructokinase, liver type;ATP-dependent 6-phosphofructokinase, liver type;ATP-PFK;PFK-B;PFK-L;phosphofructo-1-kinase isozyme B;phosphofructokinase 1;phosphofructokinase, liver, B-type;phosphohexokinase.
• NCBI Gene Id: 25741
• Protein Name: P30835
• Description of Target: ATP-dependent 6-phosphofructokinase, liver type
• Uniprot ID: https://www.uniprot.org/uniprot/P30835
• Protein Accession #: NP_037322.1
• Nucleotide Accession #: NM_013190.4
• Protein Size (# AA): 780
• Molecular Weight: 85 kDa