PFKL Antibody (OAGA00422)

Catalog No: OAGA00422

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Hep G2
7.5% SDS PAGE
OAGA00422 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using PFKL(OAGA00422) antibody at 1:500 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed A549, using PFKL(OAGA00422) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid 0.1M Tris-Glycine (pH 7) with 10% glycerol and 0.01% Thimerosal
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
  Positive Controls: A431, H1299, HeLa, HepG2
• Reconstitution and Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze/thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PFKL. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: Varies by lot. See vial for concentration.
• Application Info: WB: 1:500~3000
  ICC/IF: 1:100~1000
  IHC-P: 1:100~1000
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: PFKL
• Gene Full Name: 6-phosphofructokinase, liver type
• Alias Symbols: PFK-B, PFK-L, ATP-PFK
• NCBI Gene Id: 5211
• Protein Name: 6-phosphofructokinase, liver type
• Description of Target: Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. The protein encoded by this gene represents the L subunit. Alternate splicing results in two transcript variants, one of which is a candidate for nonsense-mediated decay (NMD).
• Uniprot ID: P17858
• Protein Accession #: NP_002617.3
• Molecular Weight: 85 kDa