PF4 ELISA Kit (Rat) (OKDD00622)

Catalog No: OKDD00622 (Formerly GWB-KBBFQ3)

Size:96 Wells

Rat PF4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked immunosorbent assay
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology PF4 ELISA Kit (Rat) (OKDD00622) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for PF4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PF4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PF4 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility:

  Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively.

  Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate.

  Mean Intra-Assay: CV<10%

  Mean Inter-Assay: CV<12%

• ELISA Kit Component:
  • Anti-PF4 Microplate: 96 Wells (12 x 8 Well Strips)
  • PF4 Lyophilized Standard: 2 x 10 ng
  • Standard Diluent: 1 x 20 mL
  • 100X PF4 Biotinylated Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Detection Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Detection Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids.
• Sensitivity: < 0.128 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of PF4. No significant cross-reactivity or interference between PF4 and analogues was observed.
• Assay Info: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Pf4
• Gene Full Name: platelet factor 4
• Alias Symbols: chemokine (C-X-C motif) ligand 4;C-X-C motif chemokine 4;Cxcl4;PF-4;Pf4a;platelet factor 4;RATPF4A.
• NCBI Gene Id: 360918
• Protein Name: Platelet factor 4
• Description of Target: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation.
• Uniprot ID: P06765
• Protein Accession #: NP_001007730.1
• Nucleotide Accession #: NM_001007729.1
• Protein Size (# AA): 105
• Molecular Weight: 11 kDa