Catalog No: OKCD02796
Size:96WELLS
Price: $725.00
SKU
OKCD02796
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Datasheets/ManualsPrintable datasheet for PF4 ELISA Kit (Bovine) (OKCD02796)
Product Info
Predicted Species ReactivityBos taurus|Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)80-93%87-105%80-90%78-101%
EDTA Plasma (n=5)78-104%78-103%82-91%97-105%
Heparin Plasma (n=5)94-102%99-105%87-95%97-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Factor 4 (PF4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Factor 4 (PF4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Factor 4 (PF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Platelet Factor 4 (PF4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range6.25-400pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10293
EDTA Plasma (n=5)84-9389
Heparin Plasma(n=5)90-9794
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Factor 4 (PF4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Factor 4 (PF4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PF4 Microplate96 Wells (12 x 8 Well strips)
PF4 Lyophilized Standard2
100X Biotinylated PF4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, platelet-poor plasma, tissue homogenates, cell lysates, cell culture cell supernatant and other biological fluids
Sensitivity2.95 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Platelet Factor 4 (PF4). No significant cross-reactivity or interference between Platelet Factor 4 (PF4) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolPF4
Gene Full Namepyruvate carboxylase
Alias SymbolsC-X-C motif chemokine 4.
Protein NamePlatelet factor 4
Description of TargetReleased during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation.
Uniprot IDP02777
Protein Accession #NP_001094532.1
Nucleotide Accession #NM_001101062.1
Protein Size (# AA)88
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