Catalog No: OKEH01690
Size:96T
Price: $525.00
SKU
OKEH01690
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PDLIM1 ELISA Kit (Human) (OKEH01690) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PDLIM1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PDLIM1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PDLIM1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 1%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.3% (n = 20)
Mean Inter-assay CV%: < 7.5% (n = 20)
ELISA Kit Component
ComponentAmount
PDLIM1 Microplate96 Wells (12 x 8 Well strips)
PDLIM1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated PDLIM1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.03 ng/mL
SpecificityNatural and recombinant Human PDZ and LIM domain protein 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPDLIM1
Gene Full NamePDZ and LIM domain 1
Alias Symbolscarboxyl terminal LIM domain protein 1;CLIM1;CLP36;CLP-36;C-terminal LIM domain protein 1;elfin;epididymis secretory protein Li 112;hCLIM1;HEL-S-112;LIM domain protein CLP-36;PDZ and LIM domain protein 1.
NCBI Gene Id9124
Protein NameO00151
Description of TargetPDZ and LIM domain protein 1
Uniprot IDhttps://www.uniprot.org/uniprot/O00151
Protein Accession #NP_066272.1
Nucleotide Accession #NM_020992.3
Protein Size (# AA)329
Molecular Weight36 kDa
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