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Catalog No: OKCD00358 (Formerly GWB-KBBHW5)
Size:96WELLS
Price: $675.00
SKU
OKCD00358
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Datasheets/ManualsPrintable datasheet for PDGFRL ELISA Kit (Human) (OKCD00358)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)80-105%90-101%94-102%95-102%
EDTA plasma(n=5)93-104%89-96%85-99%98-105%
heparin plasma(n=5)78-98%93-101%92-101%79-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor Receptor Like Protein (PDGFRL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Derived Growth Factor Receptor Like Protein (PDGFRL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Derived Growth Factor Receptor Like Protein (PDGFRL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Platelet Derived Growth Factor Receptor Like Protein (PDGFRL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range3.12-200ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)93-10196
EDTA plasma(n=5)89-10397
heparin plasma(n=5)97-105102
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Derived Growth Factor Receptor Like Protein (PDGFRL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Derived Growth Factor Receptor Like Protein (PDGFRL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PDGFRL Microplate96 Wells (12 x 8 Well Strips)
PDGFRL Lyophilized Standard2 x 10 ng
100X Biotinylated PDGFRL Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids.
Sensitivity< 0.071 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Platelet Derived Growth Factor Receptor Like Protein (PDGFRL). No significant cross-reactivity or interference between Platelet Derived Growth Factor Receptor Like Protein (PDGFRL) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolPDGFRL
Gene Full Nameplatelet derived growth factor receptor like
Alias SymbolsPDGF receptor beta-like tumor suppressor;PDGFR-like protein;PDGRL;platelet-derived growth factor receptor-like protein;platelet-derived growth factor-beta-like tumor suppressor;PRLTS.
NCBI Gene Id5157
Protein NamePlatelet-derived growth factor receptor-like protein
Description of TargetPlatelet-derived growth factor receptor-like protein
Uniprot IDQ15198
Protein Accession #NP_006198
Nucleotide Accession #NM_006207.2
Protein Size (# AA)375
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