PDE4D Antibody (OAGA00330)

Catalog No: OAGA00330

Size:100 ul

Sample (30 ug of whole cell lysate)
A: A431
B: HeLa
7.5% SDS PAGE
OAGA00330 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using PDE4D(OAGA00330) antibody at 1:100 dilution.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using PDE4D(OAGA00330) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with(Red) at 1:500.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. In 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Positive Controls: A431, HeLa
  Cellular Localization: Cytoplasm (By similarity), Membrane (By similarity), Cytoplasm, cytoskeleton (By similarity), Centrosome (By similarity)
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PDE4D. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.3 mg/ml
• Application Info: Suggested starting dilutions are as follows:
  WB: 1:500-1:3000,
  IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)],
  ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
  Positive Controls: A431 , HeLa
• Publications:

  Inactivation of oncogenic cAMP-specific phosphodiesterase 4D by miR-139-5p in response to p53 activation. Elife. 5, (2016). 27383270

Target Info

• Gene Symbol: PDE4D
• Gene Full Name: cAMP-specific 3',5'-cyclic phosphodiesterase 4D
• Alias Symbols: DPDE3, PDE43, STRK1, ACRDYS2, HSPDE4D, PDE4DN2
• NCBI Gene Id: 5144
• Protein Name: cAMP-specific 3',5'-cyclic phosphodiesterase 4D
• Description of Target: The PDE4D gene is complex and has at least 9 different isoforms that encode functional proteins. These proteins degrade the second messenger cAMP, which is a key signal transduction molecule in multiple cell types, including vascular cells (Dominiczak and McBride, 2003 [PubMed 14517535]).[supplied by OMIM]
• Uniprot ID: Q08499
• Protein Accession #: NP_001098101.1
• Molecular Weight: 91 kDa