PDE10A ELISA Kit (Human) (OKEH04648)

Catalog No: OKEH04648

Size:96T

Human PDE10A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology PDE10A ELISA Kit (Human) (OKEH04648) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PDE10A has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for PDE10A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PDE10A captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • Anti-PDE10A Microplate: 96 Wells (12 x 8 Well Strips)
  • PDE10A Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • PDE10A Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.18 ng/mL
• Specificity: Natural and recombinant Human cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PDE10A
• Gene Full Name: phosphodiesterase 10A
• Alias Symbols: 3',5' cAMP/cGMP phosphodiesterase;ADSD2;cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A;dJ416F21.1 (phosphodiesterase 10A);HSPDE10A;IOLOD;LINC00473;PDE10A19;phosphodiesterase 10A1 (PDE10A1);phosphodiesterase 10A7 (PDE10A7).
• NCBI Gene Id: 10846
• Protein Name: Q9Y233
• Description of Target: cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A
• Uniprot ID: https://www.uniprot.org/uniprot/Q9Y233
• Protein Accession #: NP_001124162.1
• Nucleotide Accession #: NM_001130690.2
• Protein Size (# AA): 799
• Molecular Weight: 88 KDa