Catalog No: OKCD00255 (Formerly GWB-KBBKA1)
Size:96WELLS
Price: $650.00
SKU
OKCD00255
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Datasheets/ManualsPrintable datasheet for PDCD1LG2 ELISA Kit (Human) (OKCD00255)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)78-98%78-91%78-101%86-94%
EDTA plasma(n=5)78-92%85-99%96-104%88-96%
heparin plasma(n=5)91-98%91-99%89-98%84-104%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Programmed Cell Death Protein 1 Ligand 2 (PDL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Programmed Cell Death Protein 1 Ligand 2 (PDL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Programmed Cell Death Protein 1 Ligand 2 (PDL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Programmed Cell Death Protein 1 Ligand 2 (PDL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)84-9895
EDTA plasma(n=5)78-9684
heparin plasma(n=5)83-9790
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Programmed Cell Death Protein 1 Ligand 2 (PDL2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Programmed Cell Death Protein 1 Ligand 2 (PDL2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PDCD1LG2 Microplate96 Wells (12 x 8 Well Strips)
PDCD1LG2 Lyophilized Standard2 x 10 ng
100X Biotinylated PDCD1LG2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.056 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Programmed Cell Death Protein 1 Ligand 2 (PDCD1LG2). No significant cross-reactivity or interference between Programmed Cell Death Protein 1 Ligand 2 (PDCD1LG2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolPDCD1LG2
Gene Full Nameprogrammed cell death 1 ligand 2
Alias SymbolsB7 dendritic cell molecule;B7DC;B7-DC;bA574F11.2;Btdc;butyrophilin B7-DC;CD273;PD-1-ligand 2;PDCD1 ligand 2;PDCD1L2;PDL2;PD-L2;programmed cell death 1 ligand 2;programmed death ligand 2.
NCBI Gene Id80380
Protein NameProgrammed cell death 1 ligand 2
Description of TargetInvolved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production (By similarity).
Uniprot IDQ9BQ51
Protein Accession #NP_079515
Nucleotide Accession #NM_025239.3
Protein Size (# AA)273
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