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Catalog No: OKCD00932 (Formerly GWB-KBBLB8)
Size:96WELLS
Price: $675.00
SKU
OKCD00932
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Datasheets/ManualsPrintable datasheet for PCSK1 ELISA Kit (Human) (OKCD00932)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)90-104%79-92%84-91%78-95%
EDTA plasma(n=5)94-101%84-102%92-101%95-102%
heparin plasma(n=5)92-99%89-103%88-105%95-103%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)97-104101
EDTA plasma(n=5)85-9388
heparin plasma(n=5)87-10496
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PCSK1 Microplate96 Wells (12 x 8 Well Strips)
PCSK1 Lyophilized Standard2 x 20 ng
100X Biotinylated PCSK1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.115 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1). No significant cross-reactivity or interference between Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolPCSK1
Gene Full Nameproprotein convertase subtilisin/kexin type 1
Alias SymbolsBMIQ12;NEC1;neuroendocrine convertase 1;PC1;PC3;prohormone convertase 1;prohormone convertase 3;Proprotein convertase 1;SPC3.
NCBI Gene Id5122
Protein NameNeuroendocrine convertase 1
Description of TargetInvolved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP.
Uniprot IDP29120
Protein Accession #NP_058787
Nucleotide Accession #NM_017091.2
Protein Size (# AA)753
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