PCNA Antibody - C-terminal region (OAAB01561)

Catalog No: OAAB01561

Size:400ul

Western blot analysis of PCNA Antibody (Center) in Jurkat, Hela, 293 cell lysates (35ug/lane). PCNA (arrow) was detected using the purified Pab.

PCNA is strongly supported by BioGPS gene expression data to be expressed in HEK293, Hela, Jurkat

Western blot analysis of PCNA (arrow) using rabbit polyclonal PCNA Antibody (C-term) either nontransfected (Lane 1) or lysates transiently transfected transfected (Lane 2) with the PCNA gene.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PCNA antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC-P, IF
• Additional Information: Function: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
  Cellular Location: Nucleus. Note=Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This PCNA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-261 amino acids from the C-terminal region of human PCNA.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Predicted Homology Based on Immunogen Sequence: Bovine, Hamster, Monkey, Rat, Rabbit
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  IF: 1:10~50

Target Info

• Gene Symbol: PCNA
• Alias Symbols: ATLD2
• NCBI Gene Id: 5111
• Protein Name: Proliferating cell nuclear antigen
• Description of Target: PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.
  
• Uniprot ID: P12004
• Protein Accession #: NP_002583.1
• Molecular Weight: 28769 Da