PCDHB16 ELISA Kit (Human) (OKCD00415)

Catalog No: OKCD00415 (Formerly GWB-KBBSP1)

Size:96WELLS

Human PCDHB16 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protocadherin Beta 16 (PCDHb16). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protocadherin Beta 16 (PCDHb16). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protocadherin Beta 16 (PCDHb16), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Protocadherin Beta 16 (PCDHb16) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protocadherin Beta 16 (PCDHb16) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protocadherin Beta 16 (PCDHb16) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PCDHB16 Microplate: 96 Wells (12 x 8 Well Strips)
  • PCDHB16 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated PCDHB16 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Protocadherin Beta 16 (PCDHb16). No significant cross-reactivity or interference between Protocadherin Beta 16 (PCDHb16) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: PCDHB16
• Gene Full Name: protocadherin beta 16
• Alias Symbols: cadherin ME1;ME1;PCDH3X;PCDHB8a;PCDH-BETA16;protocadherin beta 8a;protocadherin beta-16;protocadherin-3x.
• NCBI Gene Id: 57717
• Protein Name: Protocadherin beta-16
• Description of Target: Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.
• Uniprot ID: Q9NRJ7
• Protein Accession #: NP_066008
• Nucleotide Accession #: NM_020957.3
• Protein Size (# AA): 776