PAM ELISA Kit (Human) (OKCD00762)

Catalog No: OKCD00762 (Formerly GWB-KBBLT4)

Size:96WELLS

Human PAM ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	96-105%	82-89%	82-96%	88-98%
  EDTA plasma(n=5)	90-105%	90-97%	87-101%	84-91%
  heparin plasma(n=5)	89-101%	91-105%	80-93%	87-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peptidylglycine Alpha Amidating Monooxygenase (PAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peptidylglycine Alpha Amidating Monooxygenase (PAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peptidylglycine Alpha Amidating Monooxygenase (PAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Peptidylglycine Alpha Amidating Monooxygenase (PAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.2-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	97-105	101
  EDTA plasma(n=5)	86-96	93
  heparin plasma(n=5)	87-95	90

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peptidylglycine Alpha Amidating Monooxygenase (PAM) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peptidylglycine Alpha Amidating Monooxygenase (PAM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PAM Microplate: 96 Wells (12 x 8 Well Strips)
  • PAM Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated PAM Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, cerebrospinal fluid and other biological fluids.
• Sensitivity: < 13.5 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Peptidylglycine Alpha Amidating Monooxygenase (PAM). No significant cross-reactivity or interference between Peptidylglycine Alpha Amidating Monooxygenase (PAM) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: PAM
• Gene Full Name: peptidylglycine alpha-amidating monooxygenase
• Alias Symbols: PAL;pancreatic peptidylglycine alpha-amidating monooxygenase;peptidyl alpha-amidating enzyme;peptidyl-alpha-hydroxyglycine alpha-amidating lyase;peptidylamidoglycolate lyase;peptidylglycine 2-hydroxylase;peptidyl-glycine alpha-amidating monooxygenase;peptidylglycine alpha-hydroxylating monooxygenase;PHM.
• NCBI Gene Id: 5066
• Protein Name: Peptidyl-glycine alpha-amidating monooxygenase
• Description of Target: Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.
• Uniprot ID: P19021
• Protein Accession #: NP_000910
• Nucleotide Accession #: NM_000919.3
• Protein Size (# AA): 973