PALB2 Antibody - middle region (OAAB07933)

Catalog No: OAAB07933

Size:400ul

PALB2 Antibody (Center) (Cat. #OAAB07933) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PALB2 antibody detected the PALB2 protein (arrow).

PALB2 is strongly supported by BioGPS gene expression data to be expressed in HepG2

PALB2 Antibody (Center) (Cat. #OAAB07933) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, FC
• Additional Information: Function: Plays a critical role in homologous recombination repair (HRR) through its ability to recruit BRCA2 and RAD51 to DNA breaks. Serves as the molecular scaffold in the formation of the BRCA1-PALB2-BRCA2 complex which is essential for homologous recombination. Strongly stimulates the DNA strand-invasion activity of RAD51, stabilizes the nucleoprotein filament against a disruptive BRC3-BRC4 polypeptide and helps RAD51 to overcome the suppressive effect of replication protein A (RPA). Functionally cooperates with RAD51AP1 in promoting of D-loop formation by RAD51. Essential partner of BRCA2 that promotes the localization and stability of BRCA2. Also enables its recombinational repair and checkpoint functions of BRCA2. May act by promoting stable association of BRCA2 with nuclear structures, allowing BRCA2 to escape the effects of proteasome-mediated degradation. Binds DNA with high affinity for D loop, which comprises single-stranded, double-stranded and branched DNA structures.
  Cellular Location: Nucleus. Note=Colocalizes with BRCA2 in nuclear foci.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This PALB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 546-574 amino acids from the Central region of human PALB2.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: 0.45 mg/ml
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  FC: 1:10~50

Target Info

• Gene Symbol: PALB2
• Alias Symbols: FANCN, PNCA3
• NCBI Gene Id: 79728
• Protein Name: Partner and localizer of BRCA2
• Description of Target: This gene encodes a protein that may function in tumor suppression. This protein binds to and colocalizes with the breast cancer 2 early onset protein (BRCA2) in nuclear foci and likely permits the stable intranuclear localization and accumulation of BRCA2.
• Uniprot ID: Q86YC2
• Protein Accession #: NP_078951.2
• Molecular Weight: 131 kDa