PAK6 Antibody - middle region (OAAB08405)

Catalog No: OAAB08405

Size:400ul

PAK6 Antibody (Center) (Cat. #OAAB08405) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PAK6 antibody detected the PAK6 protein (arrow).

PAK6 Antibody (Center) (Cat. #OAAB08405)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PAK6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of PAK6 Antibody (Center)(Cat#OAAB08405) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC, IF
• Additional Information: Precautions: PAK6 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Serine/threonine protein kinase that plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including AR or MAP2K6/MAPKK6. Phosphorylates the DNA-binding domain of androgen receptor/AR and thereby inhibits AR-mediated transcription. Inhibits also ESR1-mediated transcription. May play a role in cytoskeleton regulation by interacting with IQGAP1. May protect cells from apoptosis through phosphorylation of BAD
  Cellular Location: Cytoplasm. Nucleus. Note=Cotranslocates into nucleus with AR in response to androgen induction
  Tissue Location: Selectively expressed in brain and testis, with lower levels in multiple tissues including prostate and breast
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This PAK6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 268-297 amino acids from the Central region of human PAK6.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  IF: 1:10~50

Target Info

• Gene Symbol: PAK6
• Alias Symbols: PAK5
• NCBI Gene Id: 56924
• Protein Name: Serine/threonine-protein kinase PAK 6
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q9NQU5
• Protein Accession #: NP_001122100.1
• Molecular Weight: 74869 Da