PADI6 ELISA Kit (Human) (OKCD00477)

Catalog No: OKCD00477 (Formerly GWB-KBBLC8)

Size:96WELLS

Human PADI6 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peptidyl Arginine Deiminase Type VI (PADI6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peptidyl Arginine Deiminase Type VI (PADI6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peptidyl Arginine Deiminase Type VI (PADI6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Peptidyl Arginine Deiminase Type VI (PADI6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peptidyl Arginine Deiminase Type VI (PADI6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peptidyl Arginine Deiminase Type VI (PADI6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PADI6 Microplate: 96 Wells (12 x 8 Well Strips)
  • PADI6 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated PADI6 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.062 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Peptidyl Arginine Deiminase Type VI (PADI6). No significant cross-reactivity or interference between Peptidyl Arginine Deiminase Type VI (PADI6) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: PADI6
• Gene Full Name: peptidyl arginine deiminase 6
• Alias Symbols: hPADVI;peptidyl arginine deiminase, type VI;peptidyl arginine deiminase-like protein;peptidylarginine deiminase type 6;Peptidylarginine deiminase VI;PREMBL2;Protein-arginine deiminase type VI;protein-arginine deiminase type-6.
• NCBI Gene Id: 353238
• Protein Name: Protein-arginine deiminase type-6
• Description of Target: Catalyzes the deimination of arginine residues of proteins (By similarity). May be involved in cytoskeletal reorganization in the egg and early embryo (PubMed:27545678).
• Uniprot ID: Q6TGC4
• Protein Accession #: NP_997304
• Nucleotide Accession #: NM_207421.4
• Protein Size (# AA): 694