Catalog No: OKCD00476 (Formerly GWB-KBBCF7)
Size:96WELLS
Price: $675.00
SKU
OKCD00476
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Datasheets/ManualsPrintable datasheet for PADI3 ELISA Kit (Human) (OKCD00476)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)84-91%83-98%85-93%78-99%
EDTA plasma(n=5)80-99%85-98%80-101%78-99%
heparin plasma(n=5)89-96%86-101%83-102%92-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peptidyl Arginine Deiminase Type III (PADI3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peptidyl Arginine Deiminase Type III (PADI3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peptidyl Arginine Deiminase Type III (PADI3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Peptidyl Arginine Deiminase Type III (PADI3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.78-50ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)94-10497
EDTA plasma(n=5)78-9584
heparin plasma(n=5)86-10298
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peptidyl Arginine Deiminase Type III (PADI3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peptidyl Arginine Deiminase Type III (PADI3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PADI3 Microplate96 Wells (12 x 8 Well Strips)
PADI3 Lyophilized Standard2 x 10 ng
100X Biotinylated PADI3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.063 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Peptidyl Arginine Deiminase Type III (PADI3). No significant cross-reactivity or interference between Peptidyl Arginine Deiminase Type III (PADI3) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolPADI3
Gene Full Namepeptidyl arginine deiminase 3
Alias SymbolsPAD3;PDI3;peptidyl arginine deiminase, type III;peptidylarginine deiminase III;protein-arginine deiminase type III;protein-arginine deiminase type-3;UHS1.
NCBI Gene Id51702
Protein NameProtein-arginine deiminase type-3
Description of TargetCatalyzes the deimination of arginine residues of proteins.
Uniprot IDQ9ULW8
Protein Accession #NP_057317
Nucleotide Accession #NM_016233.2
Protein Size (# AA)664
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