P4HA3 ELISA Kit (Human) (OKCD00420)

Catalog No: OKCD00420 (Formerly GWB-KBBPS1)

Size:96WELLS

Human P4HA3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prolyl-4-Hydroxylase Alpha Polypeptide III (P4Ha3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prolyl-4-Hydroxylase Alpha Polypeptide III (P4Ha3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prolyl-4-Hydroxylase Alpha Polypeptide III (P4Ha3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Prolyl-4-Hydroxylase Alpha Polypeptide III (P4Ha3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prolyl-4-Hydroxylase Alpha Polypeptide III (P4Ha3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prolyl-4-Hydroxylase Alpha Polypeptide III (P4Ha3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-P4HA3 Microplate: 96 Wells (12 x 8 Well Strips)
  • P4HA3 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated P4HA3 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.066 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Prolyl-4-Hydroxylase Alpha Polypeptide III (P4Ha3). No significant cross-reactivity or interference between Prolyl-4-Hydroxylase Alpha Polypeptide III (P4Ha3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: P4HA3
• Gene Full Name: prolyl 4-hydroxylase subunit alpha 3
• Alias Symbols: 4-PH alpha-3;collagen prolyl 4-hydroxylase alpha(III);C-P4H alpha III;procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III;Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3;prolyl 4-hydroxylase subunit alpha-3;prolyl 4-hydroxylase, alpha polypeptide III.
• NCBI Gene Id: 283208
• Protein Name: Prolyl 4-hydroxylase subunit alpha-3
• Description of Target: Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
• Uniprot ID: Q7Z4N8
• Protein Accession #: NP_001275677
• Nucleotide Accession #: NM_001288748.1
• Protein Size (# AA): 544