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Catalog No: OKEH05605
Size:96T
Price: $625.00
SKU
OKEH05605
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH05605
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology P2rx7 ELISA Kit (Mouse) (OKEH05605) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for P2rx7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for P2rx7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample P2rx7 captured in the well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 92%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <5.7%, n = 20
Mean Inter-Assay Precision - <8.1%, n = 20
ELISA Kit Component
ComponentAmount
P2RX7 Microplate96 Wells (12 x 8 Well strips)
P2RX7 Lyophilized Standard2
100X Biotinylated P2RX7 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.083 ng/mL
SpecificityNatural and recombinant Mouse P2X purinoceptor 7
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolP2rx7
Gene Full Namepurinergic receptor P2X, ligand-gated ion channel, 7
Alias SymbolsAI467586;ATP receptor;P2X;P2X purinoceptor 7;P2X(7;P2X(7);P2X7 purinoceptor;P2X7 receptor;P2X7R;P2Z receptor;Purinergic receptor.
NCBI Gene Id18439
Protein NameQ9Z1M0
Description of TargetP2X purinoceptor 7
Uniprot IDhttps://www.uniprot.org/uniprot/Q9Z1M0
Protein Accession #NP_035157.2
Nucleotide Accession #NM_011027.3
Protein Size (# AA)595
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