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P2rx7 Antibody (OASA05733)
Datasheets/Manuals | Printable datasheet for P2rx7 Antibody (OASA05733) |
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Predicted Species Reactivity | Mouse |
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Product Format | Liquid. Phosphate buffered saline |
Clonality | Monoclonal |
Clone | Hano43 |
Isotype | IgG2b |
Host | Rat |
Application | Flow cytometry|Immunofluorescence |
Additional Information | Fusion Partners: Spleen cells from immunised rats were fused with cells of the Sp2/0 myeloma cell line. |
:: | Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant Preservative Stabilisers: 0.09% Sodium Azide (NaN3) |
:: | Approx Protein Conc: IgG concentration 1.0mg/ml Buffer Solutions: Phosphate buffered saline pH7.4 |
Reconstitution and Storage | -20°C |
Immunogen | A P2X7-expression construct and a final boost with P2X7-transfected HEK cells. |
Purification | Protein G Purified |
Predicted Homology Based on Immunogen Sequence | Mouse |
Concentration | 1 mg/ml |
Specificity | OASA05733 recognises the P2X purinoceptor 7, also known as P2X7. The P2X7 ATP receptor has a distinctive long C-terminal tail with multiple potential protein and lipid interaction motifs and is highly polymorphic. It is a cation selective ion channel that opens up on binding of extracellular ATP. Sustained activation by extracellular ATP results in the formation of a reversible pore in the plasma membrane that is permeable to hydrophilic solutes of up to 900 Da. Once a pore is opened massive upset of cytoplasmic ion homeostasis occurs and the pore stays open as long as it is bound by ATP. Should ATP stimulation continue the cell will become irreversibly damaged and die. P2X7 plays a key role in the maturation and release of IL-1 and other IL-1 family members during inflammation. As such, P2X7 blockers might be useful as anti-inflammatory agents. |
Application Info | Flow Cytometry: 1/25 - 1/200 Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. |
Application Data | Application #1: Rat peritoneal macrophages stained with Rat anti Mouse P2X7 Application #2: Rat peritoneal macrophages stained with Rat anti Mouse P2X7:Alexa Fluor 488 |
Publications | Differential requirement for P2X7R function in IL-17 dependent vs. IL-17 independent cellular immune responses. Am J Transplant. 14, 1512-22 (2014). 24866539 |
Description |
Gene Symbol | P2rx7 |
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Alias Symbols | P2X, P2X(7, P2X7R, P2X(7), AI467586 |
NCBI Gene Id | 18439 |
Protein Name | P2X purinoceptor 7 |
Description of Target | RAT ANTI MOUSE P2X7 |
Uniprot ID | Q9Z1M0 |
Protein Accession # | NP_035157.2 |
Protein Size (# AA) | 595 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "P2rx7 Antibody (OASA05733)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Mouse".
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How long will it take to receive "P2rx7 Antibody (OASA05733)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "P2rx7 Antibody (OASA05733)" provided in?
This item is provided in "Liquid. Phosphate buffered saline".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "P2rx7 Antibody (OASA05733)"?
This target may also be called "P2X, P2X(7, P2X7R, P2X(7), AI467586" in publications.
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What is the shipping cost for "P2rx7 Antibody (OASA05733)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "P2rx7 Antibody (OASA05733)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "P2rx7 Antibody (OASA05733)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "P2rx7 Antibody (OASA05733)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "P2RX7"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "P2RX7"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "P2RX7"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "P2RX7"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "P2RX7"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "P2RX7"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.