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Catalog No: OKBB00250
Size:96 Tests
Price: $583.00
SKU
OKBB00250
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva's human P-Selectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for P-Selectin has been precoated onto 96-well plates. Standards(NS0, W42-A771) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for P-Selectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human P-Selectin amount of sample captured in plate.
ELISA Kit Range156 pg/ml - 10,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
Mean (pg/mL)6751105262285816573183
St. Dev.29.764.09146.822.264.52144.8
%CV4.45.85.592.583.894.54
ELISA Kit Component
ComponentAmount
Lyophilized recombinant human P-Selectin standard10 ng/tube x 2
Anti-human P-Selectin Antibody Well Plate96 Wells
Sample diluent buffer30 mL
Biotinylated anti-human P-Selectin antibody130 uL, dilution 1:100
Antibody diluent buffer12 mL
Avidin-Biotin-Peroxidase Complex (ABC)130 uL, dilution 1:100
ABC diluent buffer12 mL
TMB color developing agent10 mL
TMB stop solution10 mL
Additional InformationRange: 62.5pg/ml-4000pg/ml
::Principle: Aviva’s human human P-Selectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay
technology. A monoclonal antibody from mouse specific for P-Selectin has been precoated onto 96-well plates. Standards (NSO,W42 - A771) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for P-Selectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human P-Selectin amount of sample captured in plate.
::Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
using.1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
using.
Background: P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes.1 It is stored in secretory granules and expressed at the plasma membrane after cell activation.2 It is known to play an important role in atherosclerosis.3 The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1 (PSGL-1).4 The standard product used in this kit is recombinant human P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 730 amino acids sequence with the molecular mass of 80 KDa
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample Typecell culture supernates, serum and plasma (heparin, EDTA, citrate)
Sensitivity<5 pg/ml
Reference1. Herrmann, S.-M.; Ricard, S.; Nicaud, V.; Mallet, C.; Evans, A.; Ruidavets, J.-B.; Arveiler, D.; Luc, G.; Cambien, F.
The P-selectin gene is highly polymorphic: reduced frequency of the pro715 allele carriers in patients with
myocardial infarction. Hum. Molec. Genet. 7: 1277-1284, 1998.
2. Florian, V.; Schluter, T.; Bohnensack, R. A new member of the sorting nexin family interacts with the C-terminus of
P-selectin. Biochem. Biophys. Res. Commun. 281: 1045-1050, 2001.
3. Burger, P. C.; Wagner, D. D. Platelet P-selectin facilitates atherosclerotic lesion development. Blood 101: 2661-2666,
2003.
4. Aigner, S.; Sthoeger, ZM.; Fogel, M.; Weber E.; Zarn, J.; Ruppert, M.; Zeller, Y.; Vestweber, D.; Stahel, R.; Sammar,
M.; Altevogt, P. CD24, a mucin-type glycoprotein, is a ligand for P-selectin on human tumor cells. Blood. 1997 May
1;89(9):3385-95.
SpecificityNatural and recombinant human sP-Selectin
ImmunogenExpression system for standard: NS0; Immunogen sequence: W42-A771
DescriptionFor quantitative detection of human P-Selectin in cell culture supernatants, serum and plasma(heparin, EDTA, citrate).
Gene SymbolSELP
Gene Full Nameselectin P (granule membrane protein 140kDa, antigen CD62)
Alias SymbolsAntigen CD62, CD62, CD62 antigen like family member P, CD62 antigen-like family member P, CD62P, FLJ45155, GMP 140, GMP-140, GMP140, Granule membrane protein 140, Granule membrane protein 140kDa, Granulocyte membrane protein, GRMP, LECAM3, Leukocyte endothelial cell adhesion molecule 3, Leukocyte-endothelial cell adhesion molecule 3, LYAM3_HUMAN, P selectin, P-selectin, PADGEM, Platelet activation dependent granule external membrane protein, Platelet activation dependent granule-external membrane protein, Platelet alpha granule membrane protein, PSEL, Selectin P, Selectin P (granule membrane protein 140kDa antigen CD62), SELP
NCBI Gene Id6403
Protein NameP-selectin
Description of TargetP-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes.1 It is stored in secretory granules and expressed at the plasma membrane after cell activation.2 It is known to play an important role in atherosclerosis.3 The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1 (PSGL-1).4 The standard product used in this kit is recombinant human P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 730 amino acids sequence with the molecular mass of 80 KDa.
Uniprot IDP16109
Protein Accession #NP_002996.2
Nucleotide Accession #NM_003005.3
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