Catalog No: OKEH06568
Size:96T
Price: $525.00
SKU
OKEH06568
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology OXR1 ELISA Kit (Human) (OKEH06568) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-OXR1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated OXR1 and incubated. The OXR1 found in the sample or standards competes with the biotinylated OXR1 for limited binding sites on the immobilized anti-OXR1 antibody. Excess unbound biotinylated OXR1 and sample or standard OXR1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated OXR1 captured in the well and inversely proportional to the amount of OXR1 which was contained in the sample or standard.
ELISA Kit Range0.625-40ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 91%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Mean Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <= 8.4% (n = 20)
Mean Inter-Assay Precision - <= 11.1% (n = 20)
ELISA Kit Component
ComponentAmount
H3F3C Microplate96 Wells (12 x 8 Well strips)
H3F3C Lyophilized Standard2
100X Biotinylated H3F3C Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.327 ng/mL
SpecificityNatural and recombinant Human Oxidation resistance protein 1
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolOXR1
Gene Full Nameoxidation resistance 1
Alias SymbolsCHEGDD;Nbla00307;oxidation resistance protein 1;putative protein product of Nbla00307;TBC/LysM-associated domain containing 3;TLDC3.
NCBI Gene Id55074
Protein NameQ8N573
Description of TargetOxidation resistance protein 1
Uniprot IDhttps://www.uniprot.org/uniprot/Q8N573
Protein Accession #NP_001029206
Nucleotide Accession #NM_001034034
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