Catalog No: OKCD02747
Size:96WELLS
Price: $650.00
SKU
OKCD02747
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Datasheets/ManualsPrintable datasheet for OSM ELISA Kit (Rat) (OKCD02747)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)85-92%95-102%88-97%92-101%
EDTA Plasma (n=5)84-98%82-101%92-99%88-96%
Heparin Plasma (n=5)97-105%95-102%84-96%94-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Oncostatin M (OSM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Oncostatin M (OSM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Oncostatin M (OSM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Oncostatin M (OSM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.62-1000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)89-9895
EDTA Plasma (n=5)81-10397
Heparin Plasma(n=5)80-10389
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Oncostatin M (OSM) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Oncostatin M (OSM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-OSM Microplate96 Wells (12 x 8 Well strips)
OSM Lyophilized Standard2
100X Biotinylated OSM Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity6.5 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Oncostatin M (OSM). No significant cross-reactivity or interference between Oncostatin M (OSM) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolOsm
Gene Full Nameoncostatin M
Alias Symbolsoncostatin-M.
NCBI Gene Id289747
Protein NameOncostatin-M
Description of TargetGrowth regulator. Inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells (By similarity). Uses only type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration.
Uniprot IDQ65Z15
Protein Accession #NP_001006962.1
Nucleotide Accession #NM_001006961.1
Protein Size (# AA)239
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