ORM1 ELISA Kit (Mouse) (OKCD02333)

Catalog No: OKCD02333

Size:96WELLS

Mouse ORM1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	78-104%	91-99%	78-101%	96-105%
  EDTA Plasma (n=5)	89-98%	95-102%	84-101%	95-103%
  Heparin Plasma (n=5)	96-104%	94-102%	86-101%	80-92%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Acid Glycoprotein (a1AGP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-1-Acid Glycoprotein (a1AGP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-Acid Glycoprotein (a1AGP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Alpha-1-Acid Glycoprotein (a1AGP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 62.5-4,000ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	79-97	86
  EDTA Plasma (n=5)	78-99	80
  Heparin Plasma(n=5)	95-105	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-1-Acid Glycoprotein (a1AGP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-1-Acid Glycoprotein (a1AGP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ORM1 Microplate: 96 Wells (12 x 8 Well strips)
  • ORM1 Lyophilized Standard: 2
  • 100X Biotinylated ORM1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernatant and other biological fluids
• Sensitivity: 24.5 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Alpha-1-Acid Glycoprotein (a1AGP). No significant cross-reactivity or interference between Alpha-1-Acid Glycoprotein (a1AGP) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Orm1
• Gene Full Name: orosomucoid 1
• Alias Symbols: Agp;AGP 1;Agp-1;Agp-2;alpha-1-acid glycoprotein 1;OMD 1;Orm;Orm-1;Orosomucoid-1.
• NCBI Gene Id: 18405
• Protein Name: Alpha-1-acid glycoprotein 1
• Description of Target: Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain (By similarity). Appears to function in modulating the activity of the immune system during the acute-phase reaction.
• Uniprot ID: Q60590
• Protein Accession #: NP_032794.1
• Nucleotide Accession #: NM_008768.2
• Protein Size (# AA): 207