ORC4 Antibody (OAEB02324)

Catalog No: OAEB02324 (Formerly GWB-636D52)

Size:100UG

OAEB02324 (0.5ug/ml) staining of Rat Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Additional Information: Reactivity Info: Tested: Human, Mouse; Expected: Human, Mouse, Rat, Dog, Cow
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: ORC4
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%
• Peptide Sequence: C-DVRQWATSSLSWL
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:8000.
  Western Blot: Approx 50kDa band observed in Human and Mouse Heart lysates (calculated MW of 50.4kDa according to Human NP_002543.2 and of 50.0kDa according to Mouse NP_036088.3). Recommended concentration: 0.5-2ug/ml.
  Other Notes: ChIP: reported by a customer to work very well in ChIP.
• Reference: * Johmura Y, Osada S, Nishizuka M, Imagawa M.; FAD24 acts in concert with histone acetyltransferase HBO1 to promote adipogenesis by controlling DNA replication.; J Biol Chem. 2008 Jan 25;283(4):2265-74.; Pubmed 18029353
  * Johmura Y, Osada S, Nishizuka M, Imagawa M.; FAD24 acts in concert with histone acetyltransferase HBO1 to promote adipogenesis by controlling DNA replication.; J Biol Chem 283:2265-74 (2008).; Pubmed 18029353
  * Radichev I, Kwon SW, Zhao Y, DePamphilis ML, Vassilev A.; Genetic analysis of human Orc2 reveals specific domains that are required in vivo for assembly and nuclear localization of the origin recognition complex.; J Biol Chem. 2006 Aug 11;281(32):23264-73.; Pubmed 16762929
  * Radichev I, Kwon SW, Zhao Y, DePamphilis ML, Vassilev A.; Genetic analysis of human Orc2 reveals specific domains that are required in vivo for assembly and nuclear localization of the origin recognition complex.; J Biol Chem 281:23264-73 (2006).; Pubmed 16762929
  * Todorovic V, Giadrossi S, Pelizon C, Mendoza-Maldonado R, Masai H, Giacca M.; Human origins of DNA replication selected from a library of nascent DNA.; Mol Cell. 2005 Aug 19;19(4):567-75.; Pubmed 16109380
  * Vashee S, Simancek P, Challberg MD, Kelly TJ.; Assembly of the human origin recognition complex.; J Biol Chem 2001 Jul 13;276(28):26666-73; Pubmed 11323433

Target Info

• Gene Symbol: Orc4|ORC4
• Gene Full Name: origin recognition complex subunit 4|origin recognition complex, subunit 4
• Alias Symbols: mMmOR;mMmORC4;Or;Orc4l;Orc4P;origin recognition complex subunit 4;origin recognition complex, subunit 4 homolog.
• NCBI Gene Id: 26428|295596|5000
• Protein Name: Origin recognition complex subunit 4
• Uniprot ID: O43929
• Protein Accession #: NP_002543.2
• Molecular Weight: 50.4; 40.9; 42.0