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Catalog No: OKEH03743
Size:96T
Price: $625.00
SKU
OKEH03743
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology OPRL1 ELISA Kit (Rat) (OKEH03743) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Oprl1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Oprl1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Oprl1 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit Component
ComponentAmount
Oprl1 Microplate96 Wells (12 x 8 Well strips)
Oprl1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Oprl1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity40.4 pg/mL
SpecificityNatural and recombinant Rat Nociceptin receptor
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolOprl1
Gene Full Nameopioid related nociceptin receptor 1
Alias Symbolskappa-type 3 opioid receptor;KOR3;KOR-3;LC132;MOR-C;nociceptin receptor;nociceptin receptor ORL1;OFQR;opiate receptor-like 1;opioid receptor-like 1;Oprl;ORL1;orphanin FQ receptor;orphanin FQ receptor-a;orphanin FQ receptor-b;orphanin FQ receptor-e;peptide receptor;ROR-C;seven transmembrane G protein-coupled receptor;XOR1.
NCBI Gene Id29256
Protein NameP35370
Description of TargetNociceptin receptor
Uniprot IDhttps://www.uniprot.org/uniprot/P35370
Protein Accession #NP_113757.1
Nucleotide Accession #NM_031569.4
Protein Size (# AA)367
Molecular Weight41 kDa
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