Catalog No: OKEH01784
Size:96T
Price: $600.00
SKU
OKEH01784
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology OPLAH ELISA Kit (Human) (OKEH01784) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for OPLAH has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for OPLAH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample OPLAH captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.6% (n = 20)
Mean Inter-assay CV%: < 9.4% (n = 20)
ELISA Kit Component
ComponentAmount
OPLAH Microplate96 Wells (12 x 8 Well strips)
OPLAH Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated OPLAH Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.076 ng/mL
SpecificityNatural and recombinant Human 5-oxoprolinase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolOPLAH
Gene Full Name5-oxoprolinase, ATP-hydrolysing
Alias Symbols5-Opase;5-oxo-L-prolinase;5-oxoprolinase;OPLA;OPLAHD;pyroglutamase.
NCBI Gene Id26873
Protein NameO14841
Description of Target5-oxoprolinase
Uniprot IDhttps://www.uniprot.org/uniprot/O14841
Protein Accession #NP_060040.1
Nucleotide Accession #NM_017570.4
Protein Size (# AA)1288
Molecular Weight137 kDa
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