Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH03353
Size:96T
Price: $675.00
SKU
OKEH03353
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH03353
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology OMD ELISA Kit (Human) (OKEH03353) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for OMD has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for OMD is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample OMD captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 8.4% (n = 20)
Mean Inter-assay CV%: <= 11.1% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-OMD Microplate96 Wells (12 x 8 Well strips)
OMD Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated OMD Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity39.5 pg/mL
SpecificityNatural and recombinant Human Osteomodulin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolOMD
Gene Full Nameosteomodulin
Alias Symbolskeratan sulfate proteoglycan osteomodulin;KSPG osteomodulin;OSAD;Osteoadherin;osteoadherin proteoglycan;osteomodulin;SLRR2C.
NCBI Gene Id4958
Protein NameQ99983
Description of TargetOsteomodulin
Uniprot IDhttps://www.uniprot.org/uniprot/Q99983
Protein Accession #NP_005005.1
Nucleotide Accession #NM_005014.2
Protein Size (# AA)421
Molecular Weight49 kDa
Write Your Own Review
You're reviewing:OMD ELISA Kit (Human) (OKEH03353)
Your Rating