OCT4 Antibody (OAAD00322)

Catalog No: OAAD00322

Size:0.1ml

western blot analysis using OCT4 mouse mAb against PMA treated HepG2 cell lysate (1).

Product Info

• Predicted Species Reactivity: Human
• Clonality: Monoclonal
• Clone: 3A5
• Isotype: IgG1
• Host: Mouse
• Application: WB
• Reconstitution and Storage: Store at 4C, for long term storage, store at -20C.
• Immunogen: Purified recombinant fragment of OCT4 expressed in E. Coli.
• Formulation: Ascitic fluid containing 0.03% sodium azide.
• Application Info: Western Bloting: 1/500 - 1/2000.
  ELISA: Propose dilution 1/10000.
  Not yet tested in other applications.
  Determining optimal working dilutions by titration test.
• Reference: 1. Stem Cells. 2009 Mar;27(3):543-9.
  2. Genome Biol. 2008;9(12):R172.

Target Info

• Gene Symbol: POU5F1
• Alias Symbols: OCT3, OCT4, OTF3, OTF4, OTF-3, Oct-3, Oct-4
• NCBI Gene Id: 5460
• Protein Name: POU domain, class 5, transcription factor 1
• Description of Target: Members of Oct family of transcription factors specifically interact with Octamer motif ATGCAAAT, a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. All of the members of the Oct family contain two highly conserved domains which are separated by 14-26 variable amino acids. These include the POU homeodomain and the POU-specific domain. Both are required for DNA binding and are involved in protein-protein interactions. Evidences indicate that regulation of Oct family transcription factors occurs at the level of phosphorylation.
  Oct4 is renowned as a transcription factor expressed by undifferentiated embryonic stem cells and embryonic germ cells.
• Uniprot ID: Q01860
• Molecular Weight: 39kDa