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Catalog No: OKEH01443
Size:96T
Price: $600.00
SKU
OKEH01443
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology OB ELISA Kit (Human) (OKEH01443) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human OB antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated OB and incubated. The OB found in the sample or standards competes with the biotinylated OB for limited binding sites on the immobilized anti-human OB antibody. Excess unbound biotinylated OB and sample or standard OB is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated OB captured in the well and inversely proportional to the amount of OB which was contained in the sample or standard.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 107%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.7% (n = 20)
Mean Inter-assay CV%: < 9.8% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-OB Microplate96 Wells (12 x 8 Well strips)
OB Lyophilized Standard2
Sample Diluent1 x 20 mL
100X OB-Biotin Complex1 x 60 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity10 pg/mL
SpecificityNatural and recombinant Human Obestatin
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolOB
Alias SymbolsOB
Protein NameObestatin
Description of TargetObestatin is a putative hormone that is potentially produced in the cells lining the stomach and small intestine of several mammals including humans. Obestatin was originally identified as an anorectic peptide, but its effect on food intake remains controversial.
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