Catalog No: OKEH04370
Size:96T
Price: $525.00
SKU
OKEH04370
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology OAS2 ELISA Kit (Human) (OKEH04370) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human OAS2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated OAS2 and incubated. The found in the sample or standards competes with the biotinylated OAS2 for limited binding sites on the immobilized anti-Human OAS2 antibody. Excess unbound biotinylated OAS2 and sample or standard OAS2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated OAS2 captured in the well and inversely proportional to the amount of OAS2 which was contained in the sample or standard.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 4.5% (n = 20)
Mean Inter-assay CV%: <= 8.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-OAS2 Microplate96 Wells (12 x 8 Well Strips)
OAS2 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
OAS2 Biotinylated Complex1 x 60 uL
Biotin Complex Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.12 ng/mL
SpecificityNatural and recombinant Human 2'-5'-oligoadenylate synthase 2
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolOAS2
Gene Full Name2'-5'-oligoadenylate synthetase 2
Alias Symbols(2'-5')oligo(A) synthetase 2;2-5A synthase 2;2'-5'-oligoadenylate synthase 2;2'-5'-oligoadenylate synthetase 2, 69/71kDa;p69 OAS / p71 OAS.
NCBI Gene Id4939
Protein NameP29728
Description of Target2'-5'-oligoadenylate synthase 2
Uniprot IDhttps://www.uniprot.org/uniprot/P29728
Protein Accession #NP_001027903.1
Nucleotide Accession #NM_001032731.1
Protein Size (# AA)719
Molecular Weight82 KDa
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