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Catalog No: OKEH03624
Size:96T
Price: $625.00
SKU
OKEH03624
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology O3FAR1 ELISA Kit (Mouse) (OKEH03624) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for O3far1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for O3far1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample O3far1 captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.9% (n = 20)
Mean Inter-assay CV%: <= 8.6% (n = 20)
ELISA Kit Component
ComponentAmount
O3far1 Microplate96 Wells (12 x 8 Well strips)
O3far1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated O3far1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.45 ng/mL
SpecificityNatural and recombinant Mouse Omega-3 fatty acid receptor 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolFfar4
Gene Full Namefree fatty acid receptor 4
Alias SymbolsAI552415;Ffa;Ffa4;free fatty acid receptor 4;Gpr1;Gpr120;Gpr129;G-protein coupled receptor 120;G-protein coupled receptor GT01;G-protein-coupled receptor GT01;GT01;KPG_013;O3f;O3far1;omega-3 fatty acid receptor 1;Pg;Pgr4.
NCBI Gene Id107221
Protein NameQ7TMA4
Description of TargetFree fatty acid receptor 4
Uniprot IDhttps://www.uniprot.org/uniprot/Q7TMA4
Protein Accession #NP_861413.1
Nucleotide Accession #NM_181748.2
Protein Size (# AA)361
Molecular Weight41 kDa
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