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Catalog No: OKCD00538 (Formerly GWB-KBBIL1)
Size:96WELLS
Price: $675.00
SKU
OKCD00538
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Datasheets/ManualsPrintable datasheet for NXN ELISA Kit (Human) (OKCD00538)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nucleoredoxin (NXN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nucleoredoxin (NXN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nucleoredoxin (NXN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Nucleoredoxin (NXN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nucleoredoxin (NXN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nucleoredoxin (NXN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-NXN Microplate96 Wells (12 x 8 Well Strips)
NXN Lyophilized Standard2 x 10 ng
100X Biotinylated NXN Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.063 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Nucleoredoxin (NXN). No significant cross-reactivity or interference between Nucleoredoxin (NXN) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolNXN
Gene Full Namenucleoredoxin
Alias SymbolsNRX;nucleoredoxin;nucleoredoxin 1;RRS2;TRG-4.
NCBI Gene Id64359
Protein NameNucleoredoxin
Description of TargetFunctions as a redox-dependent negative regulator of the Wnt signaling pathway, possibly by preventing ubiquitination of DVL3 by the BCR(KLHL12) complex. May also function as a transcriptional regulator act as a regulator of protein phosphatase 2A (PP2A) (By similarity).
Uniprot IDQ6DKJ4
Protein Accession #NP_001192248
Nucleotide Accession #NM_001205319.1
Protein Size (# AA)435
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