Catalog No: OKEH04604
Size:96T
Price: $600.00
SKU
OKEH04604
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Ntrk1 ELISA Kit (Mouse) (OKEH04604) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ntrk1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Ntrk1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ntrk1 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-Ntrk1 Microplate96 Wells (12 x 8 Well Strips)
Ntrk1 Lyophilized Standard2
Sample Diluent1 x 20 mL
Ntrk1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity40.3 pg/mL
SpecificityNatural and recombinant Mouse High affinity nerve growth factor receptor
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolNtrk1
Gene Full Nameneurotrophic tyrosine kinase, receptor, type 1
Alias SymbolsC80751;high affinity nerve growth factor receptor;Neurotrophic tyrosine kinase receptor type 1;Tk;Tkr;Tr;trk;TrkA.
NCBI Gene Id18211
Protein NameQ3UFB7
Description of TargetHigh affinity nerve growth factor receptor
Uniprot IDhttps://www.uniprot.org/uniprot/Q3UFB7
Protein Accession #NP_001028296.1
Nucleotide Accession #NM_001033124.1
Protein Size (# AA)799
Molecular Weight88 KDa
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