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Catalog No: OKEH06733
Size:96T
Price: $625.00
SKU
OKEH06733
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Nt5c3b ELISA Kit (Mouse) (OKEH06733) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nt5c3b has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nt5c3b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nt5c3b captured in the well.
ELISA Kit Range0.312-20ng/ml
ELISA Kit RecoveryMean recovery when spiking into serum = 93.1%
ELISA Kit ReproducibilityIntra-Assay Reproducibility CV% < 8.5% (n=20)< br>Inter-Assay Reproducibility CV% < 10.0% (n=24)
ELISA Kit Component
ComponentAmount
ZAP70 Microplate96 Wells (12 x 8 Well strips)
ZAP70 Lyophilized Standard2 x 20 ng
100X Biotinylated ZAP70 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.312 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolNt5c3b
Gene Full Name5'-nucleotidase, cytosolic IIIB
Alias Symbols2610037D24Rik;5'-nucleotidase, cytosolic III-like;7-methylguanosine nucleotidase;7-methylguanosine phosphate-specific 5'-nucleotidase;AI841843;C330027I04Rik;cN-III-like protein;cytosolic 5'-nucleotidase 3B;cytosolic 5'-nucleotidase III-like protein;N(7)-methylguanylate 5'-phosphatase;Nt5c;Nt5c3l.
NCBI Gene Id68106
Protein NameQ3UFY7
Description of Target7-methylguanosine phosphate-specific 5'-nucleotidase
Uniprot IDhttps://www.uniprot.org/uniprot/Q3UFY7
Protein Accession #NP_001096120
Nucleotide Accession #NM_001102650
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