NRAS Antibody (OAAF01913)

Catalog No: OAAF01913 (Formerly GWB-ASD183)

Size:100 ug

Western blot analysis of extracts from HeLa cells, using RASH/RASK Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using RASH/RASK Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human RASH/RASK.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: RASH/RASK/RASN antibody detects endogenous levels of total RASH/RASK/RASN protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:20000
• Reference: Akiyuki Takaya, Mol. Biol. Cell, May 2007; 18: 1850 - 1860.
  Waleed F. Khalaf, J. Immunol., Feb 2007; 178: 2527 - 2534.
  Izumi Oinuma, J. Biol. Chem., Jan 2007; 282: 303 - 318.
  Paul M. Campbell, Cancer Res., Mar 2007; 67: 2098 - 2106.

Target Info

• Gene Symbol: HRAS|KRAS|NRAS
• Gene Full Name: HRas proto-oncogene, GTPase|KRAS proto-oncogene, GTPase|NRAS proto-oncogene, GTPase
• Alias Symbols: ALPS4;C-BAS/HAS;cellular c-Ki-ras2 proto-oncogene;cellular transforming proto-oncogene;CFC2;C-HA-RAS1;c-has/bas p21 protein;C-H-RAS;c-Ki-ras;c-Ki-ras2;c-Kirsten-ras protein;C-K-RAS;'C-K-RAS;CMNS;CTLO;GTP- and GDP-binding peptide B;GTPase HRas;GTPase KRas;GTPase NRas;HAMSV;Ha-Ras;Ha-Ras1 proto-oncoprotein;Harvey rat sarcoma viral oncogene homolog;Harvey rat sarcoma viral oncoprotein;H-Ras-1;HRAS1;H-RASIDX;KI-RAS;Kirsten rat sarcoma viral oncogene homolog;Kirsten rat sarcoma viral proto-oncogene;K-Ras;K-Ras 2;K-ras p21 protein;KRAS1;KRAS2;K-RAS2A;K-RAS2B;K-RAS4A;K-RAS4B;NCMS;neuroblastoma RAS viral (v-ras) oncogene homolog;neuroblastoma RAS viral oncogene homolog;N-ras;N-ras protein part 4;NRAS1;NS;NS3;NS6;OES;oncogene KRAS2;p19 H-RasIDX protein;p21ras;PR310 c-K-ras oncogene;RALD;Ras family small GTP binding protein H-Ras;RASH1;RASK2;transformation gene: oncogene HAMSV;transforming protein N-Ras;transforming protein p21;v-Ha-ras Harvey rat sarcoma viral oncogene homolog;v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog;v-ras neuroblastoma RAS viral oncogene homolog.
• NCBI Gene Id: 3265|3845|4893
• Protein Name: GTPase HRas|GTPase KRas|GTPase NRas
• Description of Target: Involved in the activation of Ras protein signal transduction (PubMed:22821884). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:12740440, PubMed:14500341, PubMed:9020151).|Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:20949621). Plays an important role in the regulation of cell proliferation (PubMed:23698361, PubMed:22711838). Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner (PubMed:24623306).|Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
• Uniprot ID: P01111|P01112|P01116
• Protein Accession #: NP_004976.2
• Molecular Weight: 21 kDa