Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKCD02770
Size:96WELLS
Price: $750.00
SKU
OKCD02770
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for NQO1 ELISA Kit (Mouse) (OKCD02770)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NADH Dehydrogenase, Quinone 1 (NQO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NADH Dehydrogenase, Quinone 1 (NQO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NADH Dehydrogenase, Quinone 1 (NQO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NADH Dehydrogenase, Quinone 1 (NQO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NADH Dehydrogenase, Quinone 1 (NQO1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NADH Dehydrogenase, Quinone 1 (NQO1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-NQO1 Microplate96 Wells (12 x 8 Well strips)
NQO1 Lyophilized Standard2
100X Biotinylated NQO1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity0.124 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of NADH Dehydrogenase, Quinone 1 (NQO1). No significant cross-reactivity or interference between NADH Dehydrogenase, Quinone 1 (NQO1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolNqo1
Gene Full NameNAD(P)H dehydrogenase, quinone 1
Alias SymbolsAV001255;azoreductase;Di;Dia4;diaphorase 4 (NADH/NADPH);Dtd;DT-diaphorase;menadione reductase;NAD(P)H dehydrogenase (quinone);NAD(P)H dehydrogenase [quinone] 1;NAD(P)H menadione oxidoreductase 1, dioxin inducible;NAD(P)H:quinone oxidoreductase 1;NM;Nmo1;Nmo-1;Nmor1;NQ;Ox;Ox-;Ox1;Ox-1;phylloquinone reductase;QR;Qr1;quinone reductase 1.
NCBI Gene Id18104
Protein NameNAD(P)H dehydrogenase [quinone] 1
Description of TargetThe enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
Uniprot IDQ64669
Protein Accession #NP_032732.3
Nucleotide Accession #NM_008706.5
Protein Size (# AA)274
Write Your Own Review
You're reviewing:NQO1 ELISA Kit (Mouse) (OKCD02770)
Your Rating
We found other products you might like!