Catalog No: OKDD00796 (Formerly GWB-KBBMX4)
Size:96 Wells
Price: $850.00
SKU
OKDD00796
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for NPY5R ELISA Kit (Rat) (OKDD00796) |
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Predicted Species Reactivity | Rat|Rattus norvegicus | ||||||||||||||||||||||
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Application | Enzyme-linked immunosorbent assay | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~4 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology NPY5R ELISA Kit (Rat) (OKDD00796) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for NPY5R has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NPY5R is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample NPY5R captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively. Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate. Mean Intra-Assay: CV<10% Mean Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue homogenates | ||||||||||||||||||||||
Sensitivity | <0.051ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of NPY5R. No significant cross-reactivity or interference between NPY5R and analogues was observed. | ||||||||||||||||||||||
Assay Info | Quantitative Sandwich ELISA |
Gene Symbol | Npy5r |
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Gene Full Name | neuropeptide Y receptor Y5 |
Alias Symbols | neuropeptide Y receptor type 5;Neuropeptide Y5 receptor;NPY5-R;NPYR5;NPY-Y5 receptor;NPYY5-R;Y5 receptor. |
NCBI Gene Id | 25340 |
Protein Name | Neuropeptide Y receptor type 5 |
Description of Target | Receptor for neuropeptide Y and peptide YY. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Seems to be associated with food intake. Could be involved in feeding disorders. |
Uniprot ID | Q63634 |
Protein Accession # | NP_037001.1 |
Nucleotide Accession # | NM_012869.1 |
Protein Size (# AA) | 445 |
Molecular Weight | 50 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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