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Catalog No: OKCD00234 (Formerly GWB-KBBEO1)
Size:96WELLS
Price: $650.00
SKU
OKCD00234
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Datasheets/ManualsPrintable datasheet for NPTX2 ELISA Kit (Human) (OKCD00234)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)78-103%79-93%82-96%80-90%
EDTA plasma(n=5)81-88%88-96%94-101%79-103%
heparin plasma(n=5)78-96%87-95%92-101%82-94%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuronal Pentraxin II (NPTX2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuronal Pentraxin II (NPTX2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuronal Pentraxin II (NPTX2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Neuronal Pentraxin II (NPTX2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)82-9686
EDTA plasma(n=5)86-9589
heparin plasma(n=5)83-9791
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuronal Pentraxin II (NPTX2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuronal Pentraxin II (NPTX2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-NPTX2 Microplate96 Wells (12 x 8 Well Strips)
NPTX2 Lyophilized Standard2 x 10 ng
100X Biotinylated NPTX2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.062 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Neuronal Pentraxin II (NPTX2). No significant cross-reactivity or interference between Neuronal Pentraxin II (NPTX2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolNPTX2
Gene Full Nameneuronal pentraxin 2
Alias Symbolsapexin;NARP;neuronal activity-regulated pentaxin;neuronal pentraxin II;neuronal pentraxin-2;NP2;NP-II.
NCBI Gene Id4885
Protein NameNeuronal pentraxin-2
Description of TargetLikely to play role in the modification of cellular properties that underlie long-term plasticity. Binds to agar matrix in a calcium-dependent manner (By similarity).
Uniprot IDP47972
Protein Accession #NP_002514
Nucleotide Accession #NM_002523.2
Protein Size (# AA)431
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