Catalog No: OKCD00233 (Formerly GWB-KBBUJ8)
Size:96WELLS
Price: $650.00
SKU
OKCD00233
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Datasheets/ManualsPrintable datasheet for NPTX1 ELISA Kit (Human) (OKCD00233)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuronal Pentraxin I (NPTX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuronal Pentraxin I (NPTX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuronal Pentraxin I (NPTX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Neuronal Pentraxin I (NPTX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuronal Pentraxin I (NPTX1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuronal Pentraxin I (NPTX1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-NPTX1 Microplate96 Wells (12 x 8 Well Strips)
NPTX1 Lyophilized Standard2 x 10 ng
100X Biotinylated NPTX1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.054 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Neuronal Pentraxin I (NPTX1). No significant cross-reactivity or interference between Neuronal Pentraxin I (NPTX1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolNPTX1
Gene Full Nameneuronal pentraxin 1
Alias Symbolsneuronal pentraxin I;neuronal pentraxin-1;NP1;NP-I.
NCBI Gene Id4884
Protein NameNeuronal pentraxin-1
Description of TargetMay be involved in mediating uptake of synaptic material during synapse remodeling or in mediating the synaptic clustering of AMPA glutamate receptors at a subset of excitatory synapses.
Uniprot IDQ15818
Protein Accession #NP_002513
Nucleotide Accession #NM_002522.3
Protein Size (# AA)432
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