NPPC ELISA Kit (Rat) (OKEH04369)

Catalog No: OKEH04369

Size:96T

Rat NPPC ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Nppc ELISA Kit (Rat) (OKEH04369) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nppc has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Nppc is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nppc captured in the well.
• ELISA Kit Range: 7.8-500pg/mL
• ELISA Kit Component:
  • Anti-Nppc Microplate: 96 Wells (12 x 8 Well Strips)
  • Nppc Lyophilized Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • Nppc Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 4.23 pg/mL
• Specificity: Natural and recombinant Rat C-type natriuretic peptide
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Nppc
• Gene Full Name: natriuretic peptide C
• Alias Symbols: C-type natriuretic peptide;natriuretic peptide precursor C;natriuretic peptide precursor type C.
• NCBI Gene Id: 114593
• Protein Name: P55207
• Description of Target: C-type natriuretic peptide
• Uniprot ID: https://www.uniprot.org/uniprot/P55207
• Protein Accession #: NP_446202.1
• Nucleotide Accession #: NM_053750.1
• Protein Size (# AA): 126
• Molecular Weight: 13 KDa