NPPB ELISA Kit (Dog) : 96 Wells (OKEH00509)

Catalog No: OKEH00509

Size:96T

Dog NPPB ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Canis lupus, Dog
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology NPPB ELISA Kit (Dog) (OKEH00509) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Dog NPPB antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Dog NPPB and incubated. The Dog NPPB found in the sample or standards competes with the biotinylated Dog NPPB for limited binding sites on the immobilized anti-Dog NPPB antibody. Excess unbound biotinylated Dog NPPB and sample or standard Dog NPPB is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Dog NPPB captured in the well and inversely proportional to the amount of Dog NPPB which was contained in the sample or standard.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.9% (n = 20)
  Mean Inter-assay CV%: < 7.5% (n = 20)
• ELISA Kit Component:
  • Anti-NPPB Microplate: 96 Wells (12 x 8 Well strips)
  • NPPB Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X NPPB-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.067 ng/mL
• Specificity: Natural and recombinant Dog Natriuretic peptides B
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: NPPB
• Gene Full Name: natriuretic peptide B
• Alias Symbols: Gamma-brain natriuretic peptide.
• NCBI Gene Id: 487441
• Protein Name: P16859
• Description of Target: Natriuretic peptides B
• Uniprot ID: https://www.uniprot.org/uniprot/P16859
• Protein Accession #: XP_013966931.1
• Nucleotide Accession #: XM_014111456.1
• Protein Size (# AA): 140
• Molecular Weight: 15 kDa