Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH04160
Size:96T
Price: $525.00
SKU
OKEH04160
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Nppa ELISA Kit (Rat) (OKEH04160) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nppa has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nppa is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nppa captured in the well.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit Component
ComponentAmount
Anti-Nppa Microplate96 Wells (12 x 8 Well Strips)
Nppa Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
Nppa Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity15.65 pg/mL
SpecificityNatural and recombinant Rat Natriuretic peptides A
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolNppa
Gene Full Namenatriuretic peptide A
Alias SymbolsANF;ANP;atrial natriuretic factor prohormone;atrial natriuretic peptide prohormone;atriopeptigen;cardiodilatin;CDD;Natriuretic peptide precursor A (pronatriodilatin, also Anf, Pnd);natriuretic peptide precursor type A;natriuretic peptides A;Pnd;preproANF;preproANP;preproCDD-ANF;prepronatriodilatin;proANF;proANP;RATANF.
NCBI Gene Id24602
Protein NameP01161
Description of TargetNatriuretic peptides A
Uniprot IDhttps://www.uniprot.org/uniprot/P01161
Protein Accession #NP_036744.1
Nucleotide Accession #NM_012612.2
Protein Size (# AA)152
Molecular Weight17 kDa
Write Your Own Review
You're reviewing:NPPA ELISA Kit (Rat) (OKEH04160)
Your Rating
We found other products you might like!