NPPA ELISA Kit (Pig) (OKEH03823)

Catalog No: OKEH03823

Size:96T

Pig NPPA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Porcine, Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology NPPA ELISA Kit (Pig) (OKEH03823) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NPPA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NPPA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NPPA captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Component:
  • Anti-NPPA Microplate: 96 Wells (12 x 8 Well strips)
  • NPPA Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated NPPA Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 38 pg/mL
• Specificity: Natural and recombinant Pig Natriuretic peptides A
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: NPPA
• Gene Full Name: natriuretic peptide A
• Alias Symbols: ANF;ANP;atrial natriuretic factor prohormone;atrial natriuretic peptide prohormone;atriopeptigen;cardiodilatin;cardiodilatin-related peptide;CDD;CDP;natriuretic peptide precursor A;natriuretic peptides A;preproANF;preproANP;preproCDD-ANF;prepronatriodilatin;proANF;proANP.
• NCBI Gene Id: 397496
• Protein Name: P24259
• Description of Target: Natriuretic peptides A
• Uniprot ID: https://www.uniprot.org/uniprot/P24259
• Protein Accession #: NP_999425.1
• Nucleotide Accession #: NM_214260.2
• Protein Size (# AA): 150
• Molecular Weight: 16 kDa