NPM1 Antibody - C-terminal region (ARP34114_T100)

Catalog No: ARP34114_T100

WB Suggested Anti-NPM1 Antibody Titration: 1.0ug/ml
Positive Control: HepG2 cell lysateThere is BioGPS gene expression data showing that NPM1 is expressed in HepG2

Lanes:
Lane 1: 25ug MIA PaCa-2 cell lysate
Lane 2: 25ug MDA-MB-231 cell lysate
Lane 3: 25ug Huh-7 cell lysate
Primary Antibody Dilution:
1:2000
Secondary Antibody:
Anti-rabbit-HRP
Secondary Antibody Dilution:
1:5000
Gene Name:
NPM1
Submitted by:
Andrei L. Gartel, University of Illinois at Chicago

Sample Type: human cornea (frozen)
Blue: DAPI
Red: NPM1
Primary Dilution: 1:100
Image Submitted By: Geraint Parfitt
Gavin Herbert Eye Institute See Customer Feedback tab for detailed information.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human NPM1
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: Human: 100%; Mouse: 92%
• Peptide Sequence: Synthetic peptide located within the following region: DDDFDDEEAEEKAPVKKGQESFKKQEKTPKTPKGPSSVEDIKAKMQASIE
• Concentration: 1.0 mg/ml
• Blocking Peptide: For anti-NPM1 (ARP34114_T100) antibody is Catalog # AAP34114 (Previous Catalog # AAPP05587)
• Sample Type Confirmation:

  There is BioGPS gene expression data showing that NPM1 is expressed in HepG2

• Reference: Falini,B., et al., (2005) N. Engl. J. Med. 352 (3), 254-266

Target Info

• Gene Symbol: NPM1
• Gene Full Name: Nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• Alias Symbols: B23, NPM
• NCBI Gene Id: 4869
• Protein Name: Nucleophosmin
• Description of Target: NPM3 is a member of Nucleophosmin (NPM) family. NPM is a ubiquitously expressed nucleolar phosphoprotein that continuously shuttles between the nucleus and cytoplasm
• Uniprot ID: P06748
• Protein Accession #: NP_954654
• Nucleotide Accession #: NM_199185
• Protein Size (# AA): 265
• Molecular Weight: 29kDa
• Protein Interactions: COX8A; NPM2; HUWE1; UBC; PA2G4; FUS; TP53; AURKA; SUMO2; SUMO3; ELF4; STAU1; CDKN2A; LGR4; SUMO1; MDM2; RPA3; RPA2; RPA1; ERG; rev; TRMT61A; OSBP; KPNA1; LIMCH1; ARMCX3; YWHAQ; PARK2; TARDBP; TUBA1C; TAB2; CEP152; DZIP1; COPG1; STK38; COPE; PARK7; AKAP11;