NPC2 ELISA Kit (Human) (OKCD01269)

Catalog No: OKCD01269 (Formerly GWB-KBBOR9)

Size:96WELLS

Human NPC2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	79-101%	84-92%	81-89%	94-105%
  EDTA plasma(n=5)	78-97%	95-102%	93-101%	93-101%
  heparin plasma(n=5)	92-105%	80-103%	86-97%	78-92%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Niemann Pick Disease Type C2 (NPC2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Niemann Pick Disease Type C2 (NPC2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Niemann Pick Disease Type C2 (NPC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Niemann Pick Disease Type C2 (NPC2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	94-101	97
  EDTA plasma(n=5)	86-95	89
  heparin plasma(n=5)	88-102	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Niemann Pick Disease Type C2 (NPC2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Niemann Pick Disease Type C2 (NPC2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-NPC2 Microplate: 96 Wells (12 x 8 Well Strips)
  • NPC2 Lyophilized Standard: 2 x 50 ng
  • 100X Biotinylated NPC2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.29 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Niemann Pick Disease Type C2 (NPC2). No significant cross-reactivity or interference between Niemann Pick Disease Type C2 (NPC2) and analogues was observed.
• Publications:

  Hiroaki Yagi, et al. Discovery of novel biomarkers for atherosclerotic aortic aneurysm through proteomics-based assessment of disease progression. 6429 (2020). 32286426

• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Description:
  View product citations for NPC2 ELISA kit (Human) on CiteAb

Target Info

• Gene Symbol: NPC2
• Gene Full Name: NPC intracellular cholesterol transporter 2
• Alias Symbols: EDDM1;epididymal protein 1;Epididymal secretory protein E1;epididymis secretory sperm binding protein;HE1;human epididymis-specific protein 1;Niemann-Pick disease type C2 protein;NPC intracellular cholesterol transporter 2;tissue-specific secretory protein.
• NCBI Gene Id: 10577
• Protein Name: NPC intracellular cholesterol transporter 2
• Description of Target: Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. The secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport.
• Uniprot ID: P61916
• Protein Accession #: NP_006423
• Nucleotide Accession #: NM_006432.3
• Protein Size (# AA): 151