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Datasheets/Manuals | Printable datasheet for NOTCH3 Antibody (OAEB00213) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Clonality | Polyclonal |
Host | Goat |
Application | IHC |
Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
Immunogen | NOTCH3 |
Purification | Ammonium Sulfate Precipitated; Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Guinea Pig: 84%; Mouse: 100%; Rat: 100% |
Peptide Sequence | C-QLGPQPEVTPKRQ |
Concentration | 0.5 mg/ml |
Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Application Info | Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Preliminary experiments gave approx 60+70kDa bands in human brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 244kDa according to NP_000426.1. The 60+70kDa bands were successfully blocked by incubation with the immunizing peptide. |
Reference | * Dang L, Fan X, Chaudhry A, Wang M, Gaiano N, Eberhart CG.; Notch3 signaling initiates choroid plexus tumor formation.; Oncogene. 2005 Sep 26; [Epub ahead of print]; Pubmed 16186803 |
Gene Symbol | NOTCH3 |
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Gene Full Name | notch receptor 3 |
Alias Symbols | CADASIL;CADASIL1;CASIL;IMF2;LMNS;neurogenic locus notch homolog protein 3;notch 3;Notch homolog 3. |
NCBI Gene Id | 4854 |
Protein Name | Neurogenic locus notch homolog protein 3 |
Uniprot ID | Q9UM47 |
Protein Accession # | NP_000426.2 |
Molecular Weight | 244 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "NOTCH3 Antibody (OAEB00213)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "NOTCH3 Antibody (OAEB00213)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "NOTCH3 Antibody (OAEB00213)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "NOTCH3 Antibody (OAEB00213)"?
This target may also be called "CADASIL;CADASIL1;CASIL;IMF2;LMNS;neurogenic locus notch homolog protein 3;notch 3;Notch homolog 3." in publications.
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What is the shipping cost for "NOTCH3 Antibody (OAEB00213)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "NOTCH3 Antibody (OAEB00213)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "NOTCH3 Antibody (OAEB00213)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "244".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "NOTCH3 Antibody (OAEB00213)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "NOTCH3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "NOTCH3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "NOTCH3"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "NOTCH3"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "NOTCH3"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "NOTCH3"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.