Catalog No: OKEH00479
Size:96T
Price: $525.00
SKU
OKEH00479
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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NOTCH1 ELISA Kit (Human) : 96 Wells (OKEH00479)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology NOTCH1 ELISA Kit (Human) (OKEH00479) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human NOTCH1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human NOTCH1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human NOTCH1 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 7.5% (n = 20)
Mean Inter-assay CV%: < 9.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-NOTCH1 Microplate96 Wells (12 x 8 Well strips)
NOTCH1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated NOTCH11 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity49 pg/mL
SpecificityNatural and recombinant Human Neurogenic locus notch homolog protein 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolNOTCH1
Gene Full Namenotch receptor 1
Alias SymbolsAOS5;AOVD1;hN1;neurogenic locus notch homolog protein 1;notch 1;Notch homolog 1, translocation-associated;TAN1;translocation-associated notch protein TAN-1.
NCBI Gene Id4851
Protein NameP46531
Description of TargetNeurogenic locus notch homolog protein 1
Uniprot IDhttps://www.uniprot.org/uniprot/P46531
Protein Accession #NP_060087.3
Nucleotide Accession #NM_017617.4
Protein Size (# AA)2555
Molecular Weight272 kDa
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