NOSTRIN ELISA Kit (Human) (OKCD00251)

Catalog No: OKCD00251 (Formerly GWB-KBBOH7)

Size:96WELLS

Human NOSTRIN ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nitric Oxide Synthase Trafficker (NOSTRIN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nitric Oxide Synthase Trafficker (NOSTRIN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nitric Oxide Synthase Trafficker (NOSTRIN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Nitric Oxide Synthase Trafficker (NOSTRIN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nitric Oxide Synthase Trafficker (NOSTRIN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nitric Oxide Synthase Trafficker (NOSTRIN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-NOSTRIN Microplate: 96 Wells (12 x 8 Well Strips)
  • NOSTRIN Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated NOSTRIN Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.054 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Nitric Oxide Synthase Trafficker (NOSTRIN). No significant cross-reactivity or interference between Nitric Oxide Synthase Trafficker (NOSTRIN) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: NOSTRIN
• Gene Full Name: nitric oxide synthase trafficking
• Alias Symbols: BM247 homolog;DaIP2;endothelial nitric oxide synthase traffic inducer;ENOS traffic inducer;eNOS-trafficking inducer;nitric oxide synthase traffic inducer;nitric oxide synthase trafficker;nostrin;ortholog of mouse disabled 2 interacting proein 2.
• NCBI Gene Id: 115677
• Protein Name: Nostrin
• Description of Target: Multivalent adapter protein which may decrease NOS3 activity by inducing its translocation away from the plasma membrane.
• Uniprot ID: Q8IVI9
• Protein Accession #: NP_001034813
• Nucleotide Accession #: NM_001039724.3
• Protein Size (# AA): 506