NOS3 ELISA Kit (Rat) (OKEH02990)

Catalog No: OKEH02990

Size:96T

Rat NOS3 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology NOS3 ELISA Kit (Rat) (OKEH02990) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-rat Nos3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Nos3 and incubated. The Nos3 found in the sample or standards competes with the biotinylated Nos3 for limited binding sites on the immobilized anti-rat Nos3 antibody. Excess unbound biotinylated Nos3 and sample or standard Nos3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Nos3 captured in the well and inversely proportional to the amount of Nos3 which was contained in the sample or standard.
• ELISA Kit Range: 0.156-10ng/ml
• ELISA Kit Component:
  • Anti-Nos3 Microplate: 96 Wells (12 x 8 Well strips)
  • Nos3 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Nos3-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.078 ng/mL
• Specificity: Natural and recombinant Rat Nitric oxide synthase, endothelial
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Nos3
• Gene Full Name: nitric oxide synthase 3
• Alias Symbols: cNOS;constitutive NOS;EC-NOS;endothelial nitric oxide synthase 3;endothelial NOS;eNos;nitric oxide synthase 3, endothelial cell;nitric oxide synthase, endothelial;NOS type III;NOSIII.
• NCBI Gene Id: 24600
• Protein Name: Q62600
• Description of Target: Nitric oxide synthase, endothelial
• Uniprot ID: https://www.uniprot.org/uniprot/Q62600
• Protein Accession #: NP_068610.1
• Nucleotide Accession #: NM_021838.2
• Protein Size (# AA): 1202
• Molecular Weight: 133 kDa